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NHLBI - National Heart Lung and Blood Institute

PROJECT SUMMARY The production of red blood cells (RBC), known as ‘erythropoiesis’, serves as a paradigm for understanding cellular differentiation and terminal maturation. Defects in erythropoiesis or RBC function cause various forms of anemia, a health burden affecting nearly one-third of the global population. Each step of erythropoiesis, including lineage specification, proliferation, differentiation, and terminal maturation into RBCs filled with hemoglobin for oxygen transport, has unique metabolic requirements with potential opportunities for therapeutic intervention. For instance, recent evidence indicates that hemoglobinopathies can be treated by stimulating glycolysis with pyruvate kinase agonists. We demonstrated previously that glutamine synthesis is uniquely upregulated during erythropoiesis to detoxify ammonium generated by heme synthesis and that enhanced glutamine synthetase activity alleviates -thalassemia. However, the comprehensive metabolic regulation of erythropoiesis remains poorly understood, which limits therapeutic opportunities. To address this knowledge gap, we performed global metabolomic profiling, isotope tracing and transcriptome analysis of staged erythroid precursors from mouse fetal liver and bone marrow. We discovered that distinct, ontogeny-specific metabolic processes govern erythropoiesis. While glucose is the primary nutrient for bioenergetics and biosynthesis in fetal liver erythroid precursors, mitochondrial fatty acid β-oxidation (FAO) becomes more active during postnatal bone marrow erythropoiesis. Surprisingly, isotope tracing showed that long chain fatty acids are essential metabolic precursors for TCA cycle intermediates and heme during adult-type erythropoiesis. Moreover, genetic disruption of FAO enzymes caused impaired erythropoiesis and anemia in adult mice, whereas erythroid FAO was profoundly dysregulated in sickle cell disease (SCD). These findings support our central hypotheses that FAO provides essential nutrients to support erythropoietic energy demands and biosynthetic needs, including heme biosynthesis, and that dysfunctional FAO contributes to the pathophysiology of SCD. Thus, the objective of this project is to elucidate the functional and mechanistic roles of fatty acid metabolism as a new regulatory pathway in erythropoiesis and RBC disorders according to three specific aims: 1) Define the functional roles of FAO in erythropoiesis; 2) Elucidate the mechanistic roles of fatty acid metabolism in erythrocyte development and function; and 3) Determine the role of fatty acid metabolism in the pathophysiology of sickle cell disease. Our hypotheses and the feasibility of proposed studies are supported by extensive preliminary data combining orthogonal, state-of-the-art metabolic and transcriptomic approaches with orthogonal mouse and human models to analyze erythropoiesis in vivo. Now we will elucidate fatty acid metabolism as a critical metabolic regulator of normal postnatal erythropoiesis and a potential therapeutic target for SCD.

National Endowment for the Humanities

FESTIVAL AND SYMPOSIUM FOR AMERICA'S 250TH [THE PROJECT BEGINS WITH A FESTIVAL OF WEEKLY SPEAKER EVENTS FOR STUDENTS, FACULTY, AND THE GENERAL PUBLIC TO CELEBRATE THE NATION?S FOUNDING, DEEPENING THEIR UNDERSTANDING OF THE REVOLUTION, FOCUS ON ITS VALUES, MEANINGS, AND LEGACIES, AND REFLECT ON ITS CALL TO CIVIC ENGAGEMENT. THE SPEAKERS ARE SERIOUS SCHOLARS WHO WILL ATTRACT AUDIENCES BY FRAMING THEIR PRESENTATIONS AROUND "HOOKS" SUCH AS ESPIONAGE, SATURDAY NIGHT LIVE, ART, AND THE MODERN RELEVANCE OF THE FOUNDERS. SEVERAL HUMANITIES DEPARTMENTS ARE INVOLVED. THE EVENTS OCCUR NEAR AN EXHIBITION OF ORIGINAL AMERICAN REVOLUTION DOCUMENTS FROM THE UNIVERSITY?S WEINER COLLECTION WITH COMPLEMENTS FROM THE AMERICAN BATTLEFIELD TRUST. AFTER THIS PERIOD OF INTENSE REFLECTION, OUR FACULTY AND STUDENT RESEARCHERS WILL PRODUCE ORIGINAL SCHOLARSHIP AND CREATIVE WORKS ON THE REVOLUTION?S THEMES. THEY WILL RECONVENE FOR A TWO-DAY PUBLIC SYMPOSIUM UNDER THE LEADERSHIP OF A KEYNOTE SPEAKER, WITH THE WEINER COLLECTION AGAIN AT THE CENTER.]

Sundiata African American Cultural Association

Festival Sundiata is the largest African American festival in the Northwest celebrating the culture and people of African decent, through music, food, dance and artistic expression. The 2017 Festival will be held Saturday June 10th from 11:00am - 8:00pm and June 11th from noon - 6:00pm at the Seattle Center 305 Harrison Street.

Sundiata African American Cultural Association

Festival Sundiata Black Arts Fest, an annual celebration of people of African descent is scheduled for Friday June 14 through Sunday, June 16, 2019 at Seattle Center, 305 Harrison Street. The event includes, music, dance, historical exhibits and arts and crafts.

festival:festival

The festival:festival organization will host a free two day arts festival to support artists and cultural workers. It will take place on August 2, 2019 from 5 pm - 10 pm at Northwest Film Forum located at 1515 12th Ave, on August 3, 2019 from 11 am - 4 pm at Amplifier located at 901 Hiawatha Pl S, and on E Denny Wy between 10th Ave and Broadway Ave from 5 pm - 10 pm on August 2 and 3, 2019.

Fidelity Foundation

Supports education, arts, health, and community development with emphasis on the Greater Boston area.

Sea-Mar Community Health Center

Fiestas Patrias is a free two-day cultural festival where the community to comes together to celebrate Latin American arts, culture and heritage through music, dance, food and more. Fiestas Patrias begins with a community parade and festival in the predominately Latino South Park neighborhood on Saturday September 16th from 11am-6pm at 1040 S. Henderson St. followed by a community fiesta with concerts, folk dancers, children's arts and crafts, and a health fair at the Seattle Center 305 Harrison St. from noon- 9pm on Saturday September 16th and noon-6pm on Sunday September 17th.

Sea-Mar Community Health Center

Fiestas Patrias is a two-day, free admission cultural festival which provides opportunities for the Puget Sound community to come together to celebrate Latin American arts, culture, and heritage through music, dance, food and more. Fiestas Patrias will take place in South Park located at 8319 8th Avenue South on September 14th and 15th from 11:00 a.m. - 6:00 p.m. beginning with a parade. There will also be a community fiesta and health fair located at the Seattle Center at 305 Harrison Street on September 14th and 15th from noon - 9:00 p.m. This project will be completed by November 29, 2019.

Florida Division of Arts & Culture

Florida Division of Arts & Culture provides grants to support arts and cultural programming, artist fellowships, arts education, and community-based arts projects across FL.

Florida Division of Arts and Culture

Supports Florida arts and cultural organizations through general program support, specific project grants, and cultural facility improvement funding.

NIGMS - National Institute of General Medical Sciences

This proposal outlines a request to acquire a BD Biosciences FACSMelody Cell Sorter at Florida A&M University (FAMU), a state-controlled public institution. The BD FACSMelody has standard forward scatter (FSC) and side scatter (SSC) detection with nine fluorescence channels and the ability to sort 34,000 drops per second with up to 4-way sorting, enabling analysis and sorting of cell populations based on fluorescently labeled phenotypic markers and reporter gene expressions. The state-of-the-art equipment will greatly enhance research capability and capacity at FAMU, aiding several on-going NIH projects including: 1) engineering liver organoids for in vivo tissue restoration and ex-vivo drug screening, 2) enhancing efficacy of nanoparticles in pancreatic patient-derived xenografts (PDX) models, and 3) development of organoid systems tailored to probe lung cancer. The shared use equipment will improve research and biomedical activities at FAMU and promote collaborations with other biomedical researchers in Tallahassee. Aims of this project are to: (1) Acquire the BD Biosciences FACSMelody System at FAMU, (2) combine the instrument into research programs to promote and expand collaborations across disciplines between FAMU and other research-intensive institutions in Tallahassee and north Florida. This equipment item has been highly requested by the faculty, students, and staff members engaged in the areas of biomedical engineering, biology, pharmaceutical sciences, and food systems. The advanced functions of the FACSMelody are currently unavailable to FAMU researchers. Acquisition of the state-of-the-art system will foster long-term shared instrumentation use and promote collaboration between research groups across campus. As FAMU's west campus in Tallahassee's Innovation Park is currently without fluorescence-activated cell sorting (FACS) capabilities, the requested system would fill a major need to advance biomedical activities at the institution. To facilitate usage, the instrument will be housed in a newly constructed research building at the college of engineering that has a collaborative concept design. Graduate and undergraduate students in biomedical engineering, biological systems engineering, and pharmaceutics will incorporate use of equipment in their undergraduate research theses and Ph.D. dissertations. The requested system will improve workforce development, while also increasing success of external research funding, further improving the research ability of the FAMU faculty.

National Institutes of Health

This Notice of Funding Opportunity (NOFO) supports projects relevant to the NIGMS mission that focus solely on the development of technologies with potential to enable acquisition of biomedical knowledge. Projects should be justified in terms of technical innovation and utility of such technical innovation for impacting future biomedical research. Outcomes or products of the proposed project should significantly advance the current state of the art and be sufficiently characterized for application in addressing a broad range of biomedical research questions.

National Institutes of Health

This Notice of Funding Opportunity (NOFO) supports projects relevant to the NIGMS mission that focus solely on the development of technologies with potential to enable acquisition of biomedical knowledge. Projects should be justified in terms of technical innovation and utility of such technical innovation for impacting future biomedical research. Outcomes or products of the proposed project should significantly advance the current state of the art and be sufficiently characterized for application in addressing a broad range of biomedical research questions.

Fondation Carasso

Supports sustainable food systems and citizen art in France and Spain. Focus on ecological transition and cultural inclusion.

Fondazione Cariplo

Milan-based banking foundation distributing 150M+ EUR annually for environment, art, research, and social services.

Fonds de recherche du Québec

FRQSC funds research and training in social sciences, humanities, arts, and letters in Quebec.

Ford Foundation

Grants for arts organizations and artists challenging narratives and advancing social justice through creative expression.

National Park Service

Produce four main-stage productions featuring Americana; daily offerings of the one act play One Destiny highlighting first person accounts leading up to Lincoln s assassination at Ford s Theatre; teacher and student workshops featuring America s past while revealing meaningful connections to today; teacher summer institutes; on-line learning programming including virtual field trips.

Language Line Services Inc. D/B/A Language Line Solutions

Editorial and Design and Graphic and Fine Art Services

Foundation for Enhancing Communities

Foundation for Enhancing Communities ($100M in assets) supports nonprofits in PA through grants focused on education, community development, arts, health. Awards range from $1,000 to $50,000.

Freeway Park Association

The Freeway Park Association will be hosting a four season art installation that will be held at Freeway Park located at 700 Seneca Street. The Four installations are: Winter 2022 light-art installation starting in December 2022, Spring Freeway Park Bloom starting in June 2023, Summer Fountain Fest starting in July 2023 and culminate with Fall Fest starting September 2023. The event is free and open to the public.

NEI - National Eye Institute

The ocular lens serves as an excellent model for studying cell fate determination, as it consists of two distinct cell types derived from the surface ectoderm: lens epithelial and fiber cells. Lens development requires suppression of pro-neurogenic pathways, distinguishing it from other anterior placodes, such as the olfactory placode, which gives rise to neurons. Mutations in key transcription factors (TFs), including AP-2α, FOXE3, and PAX6, lead to overlapping developmental defects such as aphakia, incomplete lens separation, and fiber cell abnormalities, suggesting shared transcriptional targets and regulatory mechanisms. However, a major gap remains in understanding how FOXE3 functions as a transcriptional regulator and how its activity influences chromatin architecture to suppress pro-neurogenic gene expression during lens formation. Preliminary studies using CUT&RUN identified a de novo FOXE3-binding motif, with strong similarity to FOXD3, and revealed that FOXE3 is required to repress neurogenic gene expression in early lens cells. Additionally, RNA-seq analyses of Foxe3 mutant lenses demonstrated significant differential gene expression linked to pro-neurogenic pathways. This led to the hypothesis that FOXE3 functions as both a transcriptional activator and repressor, coordinating chromatin remodeling to establish and maintain lens cell identity. To test this, state-of-the-art chromatin and transcriptomic technologies, including single- nucleus multiomics (ATAC-seq + RNA-seq), CUT&RUN, and functional assays in chick embryos, to dissect FOXE3’s molecular mechanisms will be conducted. Aim 1 will identify FOXE3 transcriptional targets and determine how chromatin accessibility (DARs) and FOXE3-bound enhancers and promoters are altered in Foxe3 mutant lenses. This will be accomplished through the integration of single-nucleus multiomics and CUT&RUN data to map the FOXE3-dependent regulatory landscape. Aim 2 will determine the functional role of FOXE3 in cell fate decisions by (1) gain and loss of function assays in chick lens cells, (2) testing its ability to suppress neurogenesis in the developing chick retina, and (3) identifying FOXE3-interacting proteins via immunoprecipitation and mass spectrometry. The completion of these aims will provide mechanistic insights into FOXE3’s role in lens development, establish a FOXE3 DNA-binding motif, and identify targets of FOXE3 regulation. These studies aim to reveal how FOXE3 mutations contribute to congenital eye disorders such as aphakia, cataracts, microphthalmia, coloboma, and Peters anomaly. By elucidating the chromatin regulatory mechanisms underlying lens cell identity, this work has the potential to inform therapeutic strategies for human eye diseases linked to transcriptional dysregulation.

Path with Art

Path with Art's purpose for this project is to utilize the power of art to build community and to foster restoration for those who have experienced trauma. The project is two-fold: hold workshops to design, create and install five `Free Little Art Galleries`(FLAG's) and provide 100 free art kits at each site for community members. Each FLAG will be placed near Path With Art's social service partners (Yesler Terrace, DESC, Plymouth Housing, KC Drug Diversion Court, and Sound Health) in underserved neighborhoods creating low-barrier entry opportunities for art exploration, inner healing, and joyous connection. At the culmination of the project, an exhibition of all collected art works will be held at the Seattle Center. The anticipated project completion date is 11/30/2022

Hunger Intervention

Hunger Intervention Program (HIP) is partnering with Lake City Farmers Market to offer free, fun activities for children. These include arts and crafts, science exhibits, and other STEM activities. In addition, HIP will also offer cooking demos and nutrition tips at the farmers' market to showcase fresh, seasonal produce from the market. The activities are in conjunction with HIP's Summer Meals program, which addresses food insecurity and promotes healthy eating for children under the age of 18 who are at risk of going hungry when schools are closed for the summer. This year, HIP is serving summer meals at twelve locations, including the Lake City Farmers Market, which runs once a week on Thursdays. This funding request is specifically for activities at the Lake City Farmers Market for the month of August, September, and October (up to ten market days). This funding will not be used for the summer meals program.