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View full policyAcquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system
About This Grant
Project Summary We seek NIH support for the acquisition of an Asymmetric Flow Field-Flow Fractionation system coupled with Multi-Angle Light Scattering (AF4-MALS) to be housed in the Johnson Foundation Structural Biology and Biophysics Core (JFBSB Core) at the University of Pennsylvania. This platform will provide critical capabilities for the separation and label-free analysis of macromolecules and nanoparticles in solution, including lipid nanoparticles (LNPs), viral vectors (e.g., AAVs), protein-nucleic acid complexes, and phase- separated assemblies. The requested commercial instrument integrates two powerful technologies: 1. field- flow fractionation (AF4) for size-based, non-destructive separation of complex or fragile species and 2. multi- angle light scattering (MALS) for the direct measurement of molar mass, radius of gyration, hydrodynamic radius, and particle size distribution without reliance on calibration standards. Additional detectors, including differential refractive index (dRI), dynamic light scattering (QELS), and ultraviolet-visible absorbance, allow the system to rigorously quantify particle concentration, heterogeneity, and conjugation state in real time. These capabilities are essential for fully understanding the structure-function relationships of therapeutic macromolecular formulations and advancing gene delivery technologies. The proposed system will support the work of 10 NIH-funded projects in structural biology and nanomedicine. Projects will include structural optimization of LNP formulations for nucleic acid delivery, analysis of biologically relevant higher-order protein assemblies and aggregates, and separation of nucleoprotein complexes. This technology complements and enhances existing SEC-MALS, SAXS, and AUC platforms at Penn, enabling orthogonal workflows across the campus research landscape. No equivalent system currently exists at the University of Pennsylvania. The requested AF4-MALS system from Wyatt Technology offers unmatched integration with ASTRA software for advanced analysis, U.S.-based support, trade-in options for legacy systems, and the lowest risk of import-related tariff costs among evaluated vendors. The JFBSB Core, with a strong track record of S10 stewardship, will ensure broad access, expert support, and long-term sustainability. The requested instrumentation will have immediate and wide-ranging impact on federally funded research programs across Penn and its affiliated institutions.
Grant Summary
Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system is a OD - NIH Office of the Director grant providing up to $406K for university, nonprofit, healthcare org. Applications are due 2027-04-30 (open). Check eligibility and apply with FindGrants.
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Focus Areas
Eligibility
How to Apply
Up to $406K
2027-04-30
- 1Confirm your organization is eligible for Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system from OD - NIH Office of the Director, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to OD - NIH Office of the Director before the deadline.
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Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system: Frequently Asked Questions
Who is eligible for the Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system?
Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system is offered by OD - NIH Office of the Director and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system provide?
Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system provides up to $406K per award from OD - NIH Office of the Director. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system deadline?
Applications for Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system are due 2027-04-30 (open). Because deadlines can change, verify the date with the funder, OD - NIH Office of the Director, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system?
To apply for Acquisition of an asymmetric field flow fractionation-multiangle light scattering (AF4-MALS) system, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from OD - NIH Office of the Director.