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View full policyOxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection
About This Grant
Abstract The Ohio State University Comprehensive Cancer Center (OSUCCC) Genomics Shared Resource (GSR) is requesting funds to purchase an Oxford Nanopore Technologies (ONT) PromethION 24 long-read nucleic acid (DNA and RNA) sequencer. There are several innovative and exciting features of the PromethION 24 which is the “third generation” sequencing instrument that best meets the needs of OSU researchers. Features include 1: the ability to assess native DNA and RNA modifications; 2: the ability to generate long-reads of a variety of lengths from a few kb to over 2 Mb of continuous sequence; 3: the ability to perform long-read single cell sequencing from 10X Genomics single-cell libraries enabling evaluation of expression of mRNA isoforms, variants and mutations from single-cells; 4: adaptive sampling which is on-instrument targeted sequencing strategy in which nucleic acids not of interest are rejected from the pores; 5: the ability to run up to 24 flow cells synchronously or asynchronously which adds flexibility in project management and ability to run multiple types of projects at the same time as well as being able to easily accommodate larger projects. The PromethION has advantages over long-read instruments from other companies because of it being able to sequence longer (>25 kb) of contiguous sequence and the ability to directly sequence RNA and interrogate multiple different RNA modifications. Furthermore, ONT offers a variety of on-instrument and cloud-based open-source software (free to users) that range from straight-forward “point and click” user-friendly tools to more sophisticated analytical programs. There are also links to community-based GIT-hub software on the ONT website. Seven major and six minor users have current NIH-funded projects that could benefit from having this technology on site. Examples of types of projects that would benefit from access to a PromethION 24 on site include: single-cell RNA- sequencing to identify allele-specific expression and escape from X-chromosome inactivation, single-cell isoform expression, long-read sequencing of single-cell spatial (10X Visium) B-cell receptors (BCR)/T-cell receptors (TCR), long-read telomere sequencing (Telo-Seq), long-read ribosomal RNA sequencing to understand ribosomal RNA processing, long-read DNA sequencing to understand genomic complexity at the Spinal Motor Neuron (SMN) locus and its impact on phenotype, and long-read direct RNA sequencing for transcript and isoform analysis in T-cells during parasitic infection. The Promethion 24 will be housed in the GSR with administration and financial support from the OSUCCC. The instrument will be available to all OSU investigators as well as outside investigators. Oversight of the instrument will be supported by the GSR technical director who has ONT experience as well as 3+ staff with NGS library expertise. There are currently no long-read PromethION 24 sequencers available in any shared resource at OSU so this instrument fills a critical need and will support cutting-edge applications and science.
Grant Summary
Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection is a OD - NIH Office of the Director grant providing up to $434K for university, nonprofit, healthcare org. Applications are due 2027-04-30 (open). Check eligibility and apply with FindGrants.
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Eligibility
How to Apply
Up to $434K
2027-04-30
- 1Confirm your organization is eligible for Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection from OD - NIH Office of the Director, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to OD - NIH Office of the Director before the deadline.
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Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection: Frequently Asked Questions
Who is eligible for the Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection?
Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection is offered by OD - NIH Office of the Director and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection provide?
Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection provides up to $434K per award from OD - NIH Office of the Director. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection deadline?
Applications for Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection are due 2027-04-30 (open). Because deadlines can change, verify the date with the funder, OD - NIH Office of the Director, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection?
To apply for Oxford Nanospore Technologies PromethlON 24 for long-read sequencing and direct nucleic acid modification detection, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from OD - NIH Office of the Director.