Mechanisms that establish a functional stem cell niche during organogenesis
About This Grant
Project Summary/Abstract: Stem cells are required for tissue homeostasis and regeneration. Accomplishing these tasks requires intimate association with a niche, a cellular microenvironment that forms in a specialized tissue location with precise morphology to enable communication with stem cells. Niches are formed by cells that must be specified for niche identity, receive signals directing migration to the appropriate tissue compartment, and respond to those cues with changes in gene expression and cytoskeletal behavior. Studying this has proven challenging, as most niches are established during embryogenesis when the tissue is inaccessible to live imaging. Under previous GM funding, my lab work established an in vivo live imaging method to enable exploration of each of these facets in the assembling Drosophila testis niche, a tractable and conserved model. Foundational studies in the adult testis have repeatedly unveiled concepts that apply to other systems, yet before my work, we did not know how this niche formed. Our method permits direct in vivo visualization, revealing discreet steps of morphogenesis. This application will perform lab work to investigate the underlying mechanisms for each step. We ask (1) How are niche cells specified? (2) How do regulators of the cytoskeleton enable niche morphogenesis? and (3) What signals direct the location of niche assembly? My previous work showed that signals from adjacent visceral muscle (Vm) are required to assemble the testis niche during embryogenesis. In response to signals, niche cells express the transcription factor islet (mammalian ortholog, Isl1), which I found polarizes F-actin and regulates anterior niche assembly. An open question is whether F-actin polarization directly enables niche morphogenesis, or if it is polarized in consequence of niche assembly. This application will harness our in vivo imaging protocol along with an incisive optogenetic approach to test direct contributions of cytoskeletal regulators in each step of niche development. Our unpublished work supported by GM R15 funds has further shown that Vm cues induce Tbx1 ortholog org-1 expression to influence niche establishment. This proposal will define genetic regulatory mechanisms through which Tbx1 regulates niche identity and morphogenesis. Finally, our data reveal that a gonad-intrinsic, non-niche cell population is guiding niche morphogenesis in concert with signals from adjacent visceral muscle. This represents a novel mechanism for niche development, which we will uncover in this application. Our work will combine the power of Drosophila genetics with incisive assays in cellular mechanics, including live in vivo imaging, optogenetics for precise temporal manipulation of the niche cortical cytoskeleton, and laser ablation to define underlying forces driving niche and stem cell behavior. Mechanisms we unveil in this model will reveal mechanics of niche establishment required to form a compartmentalized niche with appropriate cellular architecture to enable tissue function.
Grant Summary
Mechanisms that establish a functional stem cell niche during organogenesis is a NIGMS - National Institute of General Medical Sciences grant providing up to $169K for university, nonprofit, healthcare org. Applications are due 2031-04-30 (open). Check eligibility and apply with FindGrants.
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Up to $169K
2031-04-30
- 1Confirm your organization is eligible for Mechanisms that establish a functional stem cell niche during organogenesis from NIGMS - National Institute of General Medical Sciences, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIGMS - National Institute of General Medical Sciences before the deadline.
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Mechanisms that establish a functional stem cell niche during organogenesis: Frequently Asked Questions
Who is eligible for the Mechanisms that establish a functional stem cell niche during organogenesis?
Mechanisms that establish a functional stem cell niche during organogenesis is offered by NIGMS - National Institute of General Medical Sciences and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Mechanisms that establish a functional stem cell niche during organogenesis provide?
Mechanisms that establish a functional stem cell niche during organogenesis provides up to $169K per award from NIGMS - National Institute of General Medical Sciences. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Mechanisms that establish a functional stem cell niche during organogenesis deadline?
Applications for Mechanisms that establish a functional stem cell niche during organogenesis are due 2031-04-30 (open). Because deadlines can change, verify the date with the funder, NIGMS - National Institute of General Medical Sciences, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Mechanisms that establish a functional stem cell niche during organogenesis?
To apply for Mechanisms that establish a functional stem cell niche during organogenesis, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIGMS - National Institute of General Medical Sciences.