Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models
NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development
About This Grant
Project Summary Faithful embryogenesis requires the precise coordination between embryonic and extraembryonic tissues. While recent advances in pluripotent stem cell (PSC)-derived partial or non-integrated embryo models have opened new avenues to dissect early human development, a significant limitation is that they lack extraembryonic lineages. Recently, a hPSCs-based blastocyst model, human blastoids that contains both embryonic and extraembryonic cells, has opened new avenues to dissect human blastocyst development. However, due to ethical and policy restrictions, projects using human blastoids are currently not funded by NIH. Consequently, there is a fundamental gap in knowledge in the molecular and cellular mechanisms of human embryogenesis, and how defects in this stage of human development contribute to pregnancy loss. To address these deficiencies, most recently we have established bovine embryo-derived stem cell lines including naïve-like embryonic stem cells (ESCs), trophoblast stem cells (TSCs), and extraembryonic endoderm stem cells (XENs), and developed an integrated model bovine blastoid by assembly of embryonic stem cells and extraembryonic stem cells, which is from a large animal species highly relevant to humans. Bovine blastoids resembled bovine blastocysts in terms of morphology, size, cell number, marker gene expression, and lineage composition and allocation, and could be robust produced with a high efficiency. Bovine blastoids represent a well-controlled cellular substrate to test hypotheses in a human-like embryo model system. The primary objective of this proposal is to use bovine blastoids as a proxy for the early steps of human development, to test key questions relevant to lineage crosstalk in vitro. We anticipate that this work will lead to an improved fundamental understanding of the key signaling events, transcriptional regulators and cell-cell interactions during human pre-implantation blastocyst development, which will help us understand the molecular and cellular contributors of early developmental anomaly in humans.
Grant Summary
Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models is a NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development grant providing up to $422K for university, nonprofit, healthcare org. Applications are due 2028-07-31 (open). Check eligibility and apply with FindGrants.
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Up to $422K
2028-07-31
- 1Confirm your organization is eligible for Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models from NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
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Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models: Frequently Asked Questions
Who is eligible for the Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models?
Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models is offered by NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models provide?
Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models provides up to $422K per award from NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models deadline?
Applications for Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models are due 2028-07-31 (open). Because deadlines can change, verify the date with the funder, NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models?
To apply for Dissect mechanisms of pre-implantation embryonic and extraembryonic lineage crosstalk using stem cell models, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NICHD - Eunice Kennedy Shriver National Institute of Child Health and Human Development.