A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy
NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases
About This Grant
Project Summary Diabetic Foot Ulcer (DFU) is a major complication of diabetes, often leading to lower extremity amputations. DFUs are characterized by a complex pathology resulting from peripheral vascular disease, neuropathy, and infections, all exacerbated by prolonged hyperglycemia, which collectively impede normal wound healing. Standard DFU treatments, such as surgical debridement, wound dressing, and infection management, primarily address individual aspects of the pathology, leading to only about 25% of cases achieving complete healing within 12 weeks. This highlights the limitations of current treatments and the need for innovative solutions that comprehensively address multiple aspects of DFU. Diabetes impairs mitochondrial activity, reducing cellular energy and hindering wound healing and closure. Our previous research showed that electric field stimulation significantly enhanced mitochondrial activity, ATP production, and the proliferation of human dermal fibroblasts. Additionally, nitric oxide (NO) has been shown to promote angiogenesis, prevents ischemia, and reduces inflammation and infection, as also demonstrated in our study with a NO releasing prohealing peptide amphiphile (PA) nanomatrix. However, in diabetic conditions, decreased endothelial NO synthase (eNOS) activity lowers NO levels. Thus, as a multi-targeted strategy, combining electric field stimulation with NO therapy may enhance DFU healing by stimulating cellular energy for wound closure and tissue regeneration, while also reducing complications, such as vascular disease, inflammation, and infection in hyperglycemic conditions. In this study, we will evaluate the therapeutic effects of electric field stimulation and NO therapy, investigating the healing mechanisms of diabetic wounds. To conduct this evaluation, we will develop a bio-engineered cell culture platform that mimics a 3D human skin extracellular matrix (ECM) environment, integrating electric stimulation and NO delivery. This platform will be designed using a peptide amphiphile (PA)-based NO-releasing nanomatrix coating on a highly porous 3D electrospun nanofiber scaffold with two-electrode arrangement (semi- capacitive coupling) for electric stimulation, as proposed in Specific Aim 1. In Specific Aim 2, we will evaluate the effects of combined treatments, particularly electric field stimulation, on behaviors related to wound healing, such as cell proliferation and ECM production, in diabetic skin cells derived from DFU patients under hyperglycemia using the platform. This evaluation will focus on the ATP binding purinergic (P2) receptor-mediated pathway. We will also test the combined treatment, particularly NO, to enhance diabetic endothelial activities, reduce inflammation, and inhibit DFU related bacterial growth under hyperglycemia using the platform in Specific Aim 3. Successful completion of this study will offer a novel DFU treatment strategy by delivering electric field stimulation and NO, validated with an innovative wound healing cell culture platform, enhancing current treatment methods. Additionally, it will enhance Rowan University's biomedical research capabilities and provide valuable hands-on research opportunities for undergraduate students, fostering their development as future scientists.
Grant Summary
A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy is a NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases grant providing up to $562K for university, nonprofit, healthcare org. Applications are due 2029-06-30 (open). Check eligibility and apply with FindGrants.
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Up to $562K
2029-06-30
- 1Confirm your organization is eligible for A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases before the deadline.
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A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy: Frequently Asked Questions
Who is eligible for the A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy?
A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy is offered by NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy provide?
A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy provides up to $562K per award from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy deadline?
Applications for A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy are due 2029-06-30 (open). Because deadlines can change, verify the date with the funder, NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy?
To apply for A Multi-targeted Approach for the Impaired Healing of Diabetic Foot Ulcers Through Combined Electric Field Stimulation and Nitric Oxide Therapy, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIDDK - National Institute of Diabetes and Digestive and Kidney Diseases.