The Role of APP-metabolites on Extracellular Vesicle Cargo and Function
NIA - National Institute on Aging
About This Grant
Alzheimer’s Disease (AD) is the most common form of dementia in the elderly. AD brains are characterized by senile plaques composed of amyloid beta (Aβ) and intracellular tangles composed of tau. Mutations in the Amyloid Precursor Protein (APP), the protein from which Aβ is derived, cause AD, but the precise function of APP in the brain is unclear. APP undergoes complex metabolism, and its metabolites are secreted by cells in extracellular vesicles (EVs). To study the function of APP-containing EVs, we isolated App- EVs from rat primary neuronal conditioned media and proteomic analysis identified the Valosin-containing protein (Vcp) as molecular cargo. Vcp is a segregase/molecular unfoldase, a known tau-interacting protein, and a protein mutated in AD related dementias (ADRDs). In this application, we hypothesize that AD and ADRD causing mutations alter the abundance and composition of App-EVs and that this affects the target and function of App-EVs. To test this, we have proposed three aims. The first aim will investigate the molecular mechanism of App-related cargo loading into EVs. The second aim will test the effect that App-EV biogenesis has on a rat model of tau pathology, as tau is a substrate for Vcp. The third aim will test the effect of App-EVs on Vcp mutation-induced pathology. The proposed aims will leverage the Tambini lab’s significant expertise in the generation, validation and use of AD rat knock-in models and the optimization of techniques for pure App- EV isolation to address fundamental questions about the function of APP.
Grant Summary
The Role of APP-metabolites on Extracellular Vesicle Cargo and Function is a NIA - National Institute on Aging grant providing up to $640K for university, nonprofit, healthcare org. Applications are due 2030-11-30 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $640K
2030-11-30
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- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
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The Role of APP-metabolites on Extracellular Vesicle Cargo and Function: Frequently Asked Questions
Who is eligible for the The Role of APP-metabolites on Extracellular Vesicle Cargo and Function?
The Role of APP-metabolites on Extracellular Vesicle Cargo and Function is offered by NIA - National Institute on Aging and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the The Role of APP-metabolites on Extracellular Vesicle Cargo and Function provide?
The Role of APP-metabolites on Extracellular Vesicle Cargo and Function provides up to $640K per award from NIA - National Institute on Aging. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the The Role of APP-metabolites on Extracellular Vesicle Cargo and Function deadline?
Applications for The Role of APP-metabolites on Extracellular Vesicle Cargo and Function are due 2030-11-30 (open). Because deadlines can change, verify the date with the funder, NIA - National Institute on Aging, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the The Role of APP-metabolites on Extracellular Vesicle Cargo and Function?
To apply for The Role of APP-metabolites on Extracellular Vesicle Cargo and Function, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIA - National Institute on Aging.