Engineering nanowired cardiac organoids for cardiac regeneration
About This Grant
Project Summary: Cardiovascular diseases (CVDs) such as myocardial ischemia/reperfusion (I/R) injury lead to extensive cardiomyocyte death and subsequent reduced cardiac function. Due to the human adult heart’s limited regenerative capacity, there is a significant need for exogenous cardiac function restoration post-I/R. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have emerged as a favorable cell source to restore myocardial function. While promising, the therapeutic potential of hPSC-CM transplantation is hampered by low cell survival and inadequate integration with host myocardium. Thus, our lab has developed nanowired human cardiac organoids composed hPSC-CM, primary human adult cardiac fibroblasts, endothelial cells, stromal cells, and electrically conductive silicon nanowires (e-SiNWs). Our in vivo data shows that organoids are capable of robustly engrafting and providing functional recovery (69% Fractional Shortening recovery) in I/R injured rat hearts, while using 10% of the cell dose (1E6, 1x106 cells/rat) that other hPSC-CM transplantation studies used (1E7, 1x107 cells/rat). Additionally, to alleviate major histocompatibility class (MHC) mismatching to provide a translational platform for hPSC-CM delivery, our lab developed isogenic cardiac organoids, composed of hPSC-CMs, -cardiac fibroblasts (hPSC-cFBs), and -endothelial cells (hPSC-ECs) from a single hiPSC cell line. The goals of this proposal are to 1) investigate the impact of e-SiNW geometry on isogenic organoid function, and 2) demonstrate the therapeutic efficacy of optimized nanowired isogenic cardiac organoids in a rat I/R injury model. The central hypothesis of this proposal is that engineered nanowire surface geometry will enhance interactions between host myocardium and transplanted hPSC-CMs within isogenic cardiac organoids, resulting in efficient engraftment and significant functional recovery. The innovation of this proposal is engineering e-SiNW surface roughness to optimize the efficiency of myocardial engraftment, and thus the functional recovery of I/R-injured hearts. My long-term goal is to leverage translational engineering and informatics to develop a clinically viable cardiac cell therapy heart repair. Accordingly, we will pursue the following two aims: 1) investigate the effects of e-SiNW geometry on isogenic cardiac organoid function, and 2) determine the therapeutic efficacy of optimized nanowired isogenic cardiac organoids to treat I/R-injured rat hearts and investigate e-SiNW graft-host interactions using spatial transcriptomics. The proposed research would provide a translational platform for cardiac repair with efficient engraftment.
Grant Summary
Engineering nanowired cardiac organoids for cardiac regeneration is a NHLBI - National Heart Lung and Blood Institute grant providing up to $48K for university, nonprofit, healthcare org. Applications are due 2027-12-31 (open). Check eligibility and apply with FindGrants.
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How to Apply
Up to $48K
2027-12-31
- 1Confirm your organization is eligible for Engineering nanowired cardiac organoids for cardiac regeneration from NHLBI - National Heart Lung and Blood Institute, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NHLBI - National Heart Lung and Blood Institute before the deadline.
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Engineering nanowired cardiac organoids for cardiac regeneration: Frequently Asked Questions
Who is eligible for the Engineering nanowired cardiac organoids for cardiac regeneration?
Engineering nanowired cardiac organoids for cardiac regeneration is offered by NHLBI - National Heart Lung and Blood Institute and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Engineering nanowired cardiac organoids for cardiac regeneration provide?
Engineering nanowired cardiac organoids for cardiac regeneration provides up to $48K per award from NHLBI - National Heart Lung and Blood Institute. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Engineering nanowired cardiac organoids for cardiac regeneration deadline?
Applications for Engineering nanowired cardiac organoids for cardiac regeneration are due 2027-12-31 (open). Because deadlines can change, verify the date with the funder, NHLBI - National Heart Lung and Blood Institute, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Engineering nanowired cardiac organoids for cardiac regeneration?
To apply for Engineering nanowired cardiac organoids for cardiac regeneration, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NHLBI - National Heart Lung and Blood Institute.