Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection
NIAID - National Institute of Allergy and Infectious Diseases
About This Grant
Project Summary/Abstract Staphylococcus aureus is a Gram-positive bacterium that causes a range of diseases from mild to severe, and it has been estimated that over 1 million deaths worldwide in 2019 can be attributed to this bacterium. Its ability to cause these infections relies on numerous virulence factors, including toxins, proteases, adhesins, immune- evasive proteins, and more that help in infection. These virulence factors are tightly controlled, enabling the bacteria to adjust their response for successful colonization and persistence. While strides are being made to understand how virulence factor regulation affects pathogenesis and antibiotic resistance, the individual contribution of each of these factors is largely unknown. One regulatory network in Gram-positive bacteria that contributes to gene expression is YjbH and Spx, which have been linked to stress responses and virulence in diverse bacterial species. However, few studies have identified how this system impacts virulence in S. aureus. In addition, clinical isolates have been found with mutations in yjbH, and how this impacts function and disease outcomes is unknown. This application uses two independent, yet complementary, Specific Aims to advance our understanding of how YjbH impacts the physiology of S. aureus and its clinical impact on pathogenesis and antibiotic resistance. Furthermore, it will clarify the role of YjbH in the cell since our preliminary data and several studies in other bacteria point to Spx-independent roles for YjbH, though this has not been carefully defined to date. Aim 1 will utilize a combination of genetic manipulation, RT-qPCR, quantitative hemolysis, and proteomics to determine how YjbH affects Agr activity. Aim 2 will be a combination of both in vitro and in vivo experiments assessing the mutations in YjbH found clinically and their relevance to pathogenesis and virulence factor regulations. This will be accomplished with genetic manipulation, quantitative hemolysis, and murine models of chronic lung infection and acute bacteremia. To accomplish these goals, a Sponsor and Co-Sponsor with complementary expertise in bacterial genetics and infection models will lead to robust training for the trainee in a collaborative research environment.
Grant Summary
Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection is a NIAID - National Institute of Allergy and Infectious Diseases grant providing up to $39K for university, nonprofit, healthcare org. Applications are due 2029-06-06 (open). Check eligibility and apply with FindGrants.
Focus Areas
Eligibility
How to Apply
Up to $39K
2029-06-06
- 1Confirm your organization is eligible for Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection from NIAID - National Institute of Allergy and Infectious Diseases, checking organization type, location, and any population or project requirements.
- 2Gather the required documents and information, including your organization details, project plan, and budget figures.
- 3Draft your application narrative and budget addressing the funder's priorities and review criteria. FindGrants can draft each section for you to review and edit.
- 4Review every section against the requirements checklist, then export a submission-ready application pack and submit it to NIAID - National Institute of Allergy and Infectious Diseases before the deadline.
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Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection: Frequently Asked Questions
Who is eligible for the Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection?
Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection is offered by NIAID - National Institute of Allergy and Infectious Diseases and is generally open to university, nonprofit, healthcare org. It is open to organizations nationwide unless the funder specifies otherwise. Review the specific eligibility terms before applying, since funders set their own requirements around organization type, location, and the population or project being served.
How much funding does the Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection provide?
Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection provides up to $39K per award from NIAID - National Institute of Allergy and Infectious Diseases. Actual award sizes depend on the scope of your project, available program funds, and the number of applicants, so build a budget that reflects realistic, allowable costs rather than the maximum figure.
When is the Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection deadline?
Applications for Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection are due 2029-06-06 (open). Because deadlines can change, verify the date with the funder, NIAID - National Institute of Allergy and Infectious Diseases, and give yourself enough time to prepare a complete, competitive application before the close date.
How do you apply for the Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection?
To apply for Determining the role of S. aureus YjbH clinical mutations in quorum sensing and infection, confirm your eligibility, gather the required documents, and prepare a narrative and budget that address the funder's priorities. FindGrants guides you step by step and can draft each section, then exports a submission-ready application pack for this grant from NIAID - National Institute of Allergy and Infectious Diseases.